In a 100 gram amount, raw shiitake mushrooms provide 34 kilocalories and are 90% water, 7% carbohydrates, 2% protein and less than 1% fat (table for raw mushrooms).
Raw shiitake mushrooms are rich sources (20% or more of the Daily Value, DV) of B vitamins and contain moderate levels of some dietary minerals (table).
When dried to about 10% water, the contents of numerous nutrients increase substantially.
Like all mushrooms, shiitakes produce vitamin D2 upon exposure of their internal ergosterol to ultraviolet B (UVB) rays from sunlight or broadband UVB fluorescent tubes.